Cytotoxic effect of endodontic irrigants in vitro.


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BAJRAMİ D., HOXHA V., Gorduysus O., Muftuoglu S. F., Zeybek N. D., Küçükkaya S.

Medical science monitor basic research, cilt.20, ss.22-26, 2014 (Scopus) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 20
  • Basım Tarihi: 2014
  • Doi Numarası: 10.12659/msmbr.890247
  • Dergi Adı: Medical science monitor basic research
  • Derginin Tarandığı İndeksler: Scopus
  • Sayfa Sayıları: ss.22-26
  • Anahtar Kelimeler: Sodium Hypochlorite - toxicity, Chlorhexidine - toxicity
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Cytotoxicity of root canal irrigants is important due to their close contact with host tissues. The aim of this study was to assess the cytotoxic effect of NaOCl 3%, Chx 2%, and MTAD on rat periodontal ligament fibroblasts, at 0.1 and 100 μl/mL, using WST-1 colorimetric method. Rat ligamental fibroblasts were exposed to the irrigants and their viability was assessed after 1, 24, 48, and 72 h. The measurements were determined using WST-1 assay, using a micro ELISA reader. At 100 ml/L all 3 irrigants were strongly cytotoxic, although CHX was less so than NaOCl and MTAD. At the 0.1 ml/L concentration, NaOCl and MTAD were only moderately cytotoxic, whereas Chx was highly deleterious to cell viability at all time points. There was a significant influence of the dilution rate of the substance, because the odds ratio for cell viability being over 50% was increased 51 times between the 100 ml/L and 0.1 ml/L dilutions. It seems that irrigating solutions should be used at lower concentrations to enhance cell viability.