Articular Cartilage Regeneration Utilizing Decellularized Human Placental Scaffold, Mesenchymal Stem Cells and Platelet Rich Plasma

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ÖZDEMİR E., EMET A., Hashemihesar R., Yuruker A. C. S., Kilic E., Uckan Cetinkaya D., ...More

TISSUE ENGINEERING AND REGENERATIVE MEDICINE, vol.17, no.6, pp.901-908, 2020 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 17 Issue: 6
  • Publication Date: 2020
  • Doi Number: 10.1007/s13770-020-00298-w
  • Journal Name: TISSUE ENGINEERING AND REGENERATIVE MEDICINE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Compendex, EMBASE, MEDLINE
  • Page Numbers: pp.901-908
  • Keywords: Cartilage, Placenta decellularization, Platelet-rich plasma, Mesenchymal stem cells, AUTOLOGOUS CHONDROCYTE IMPLANTATION, INTRAARTICULAR DELIVERY, OSTEOCHONDRAL DEFECTS, STROMAL CELLS, DIFFERENTIATION, TRANSPLANTATION, OSTEOARTHRITIS, BIOMATERIALS, TRACKING, REPAIR
  • Hacettepe University Affiliated: Yes

Abstract

Background: Articular cartilage repair has been a challenge in orthopedic practice due to the limited self-regenerative capability. Optimal treatment method for cartilage defects has not been defined. We investigated the effect of decellularized human placental (DHP) scaffold, mesenchymal stem cells (MSC) and platelet-rich plasma (PRP) on hyaline cartilage regeneration in a rat model. Methods: An osteochondral defect was created in trochlea region of the femur in all groups, bilaterally. No additional procedure was performed in control group (n = 14). Only the DHP scaffold was applied to the P group (n = 14). The DHP scaffold and 1 x 10(6)MSCs were applied to the PS group (n = 14). The DHP scaffold and PRP were applied to the PP group (n = 14). The DHP scaffold, 1 x 10(6)MSCs and PRP were applied to the PSP group (n = 14). Outcome measures at 12 weeks included Pineda histology score and qualitative histology. Results: The mean Pineda scores of P, PS, PP, and PSP groups were significantly better than the control group (p = 0.031,p = 0.002,p < 0.001,p < 0001, respectively). There was no statistically difference in mean Pineda scores of P, PS, PP, and PSP groups (p > 0.05). Conclusion: In conclusion, the DHP scaffold appears to be a promising scaffold on hyaline cartilage regeneration. The augmentation of DHP scaffold with MSCs and PRP combinations did not enhance its efficacy on articular cartilage regeneration.