Molecularly imprinted composite cryogels for hemoglobin depletion from human blood

Baydemir G., Andac M., Percin I., Derazshamshir A., DENİZLİ A.

JOURNAL OF MOLECULAR RECOGNITION, cilt.27, sa.9, ss.528-536, 2014 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 27 Sayı: 9
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1002/jmr.2376
  • Dergi Adı: JOURNAL OF MOLECULAR RECOGNITION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.528-536
  • Anahtar Kelimeler: protein depletion, molecularly imprinted polymers, cryogel, proteome applications, AFFINITY-CHROMATOGRAPHY, MONOLITHIC CRYOGELS, SUPERMACROPOROUS CRYOGELS, SELECTIVE REMOVAL, ALBUMIN DEPLETION, CONCANAVALIN-A, HUMAN PLASMA, ADSORPTION, POLYMERS, NANOPARTICLES
  • Hacettepe Üniversitesi Adresli: Evet

Özet

A molecularly imprinted composite cryogel (MICC) was prepared for depletion of hemoglobin from human blood prior to use in proteome applications. Poly(hydroxyethyl methacrylate) based MICC was prepared with high gel fraction yields up to 90%, and characterized by Fourier transform infrared spectrophotometer, scanning electron microscopy, swelling studies, flow dynamics and surface area measurements. MICC exhibited a high binding capacity and selectivity for hemoglobin in the presence of immunoglobulin G, albumin and myoglobin. MICC column was successfully applied in fast protein liquid chromatography system for selective depletion of hemoglobin for human blood. The depletion ratio was highly increased by embedding microspheres into the cryogel (93.2%). Finally, MICC can be reused many times with no apparent decrease in hemoglobin adsorption capacity. Copyright (C) 2014 John Wiley & Sons, Ltd.