Chiral separation-based ligand exchange by open-tubular capillary electrochromatography

Aydogan C., DENİZLİ A.

ANALYTICAL BIOCHEMISTRY, cilt.447, ss.55-57, 2014 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 447
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1016/j.ab.2013.11.006
  • Dergi Adı: ANALYTICAL BIOCHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.55-57
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Chiral ligand-exchange enantioseparation of aliphatic and aromatic amino acids was successfully performed using a new open-tubular zwitterionic column with tentacle-type polymer stationary phase. The polymeric stationary phase was prepared using 3-chloro-2-hydroxypropyl methacrylate (HPMA-Cl), a new reactive monomer. The preparation procedure of the open-tubular column included silanization, in situ graft polymerization with HPMA-Cl, and L-histidine (L-His) modification. L-His was used as a chiral ligand-exchange selector and copper(II) as a central ion. Successful enantioseparation of six pairs of amino acid enantiomers was achieved with a buffer of 5 mM CuSO4, 20 mM (NH4)(2)SO4 at pH 3.0. (C) 2013 Elsevier Inc. All rights reserved.