Preparation and in vitro evaluation of chitosan nanoparticles containing a caspase inhibitor
INTERNATIONAL JOURNAL OF PHARMACEUTICS, vol.298, no.2, pp.378-383, 2005 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 298 Issue: 2
- Publication Date: 2005
- Doi Number: 10.1016/j.ijpharm.2005.03.027
- Journal Name: INTERNATIONAL JOURNAL OF PHARMACEUTICS
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.378-383
- Keywords: chitosan, nanoparticles, Z-DEVD-FMK, HPLC, PEGYLATED POLYCYANOACRYLATE NANOPARTICLES, BRAIN, DELIVERY, CARRIERS
- Hacettepe University Affiliated: Yes
Abstract
The aim of this work was to develop a formulation for Z-DEVD-FMK, a peptide which is a caspase inhibitor and has been used in experimental animal studies for a decade. Peptide loaded chitosan nanoparticles were obtained by ionotropic gelation process and Z-DEVD-FMK was quantified by an HPLC method. The influence of the initial peptide concentration on the nanoparticle characteristics and release behavior was evaluated. The CS nanoparticles have a particle diameter (Z-average) ranging from approximately 313-412 nm and a positive zeta potential (20-28 mV). The formulation with the initial peptide concentration of 400 ng/ml provided the highest loading capacity (0.46%) and the highest extent of release (65% at 24 h) suggesting the possibility to achieve a therapeutic dose. According to the data obtained, this chitosan-based nanotechnology opens new and interesting perspectives for anticaspase activity. (c) 2005 Elsevier B.V. All rights reserved.