P-31 NMR correlation maps of O-18/O-16 chemical shift isotopic effects for phosphometabolite labeling studies

Creative Commons License

Juranic N., NEMUTLU E., Zhang S., Dzeja P., Terzic A., Macura S.

JOURNAL OF BIOMOLECULAR NMR, cilt.50, sa.3, ss.237-245, 2011 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 50 Sayı: 3
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1007/s10858-011-9515-3
  • Dergi Adı: JOURNAL OF BIOMOLECULAR NMR
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.237-245
  • Anahtar Kelimeler: P-31 NMR chemical shift correlation, O-18/O-16 isotopic effects, ATP turnover, Isotopologue distribution, Oxygen-18, High-energy phosphoryl dynamics, Phosphotransfer, Phosphometabolomics, KNOCKOUT HEART, ENERGETICS, PHOSPHOTRANSFER, MECHANISM, EXCHANGE, O-18
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Intramolecular correlations among the O-18-labels of metabolic oligophosphates, mapped by J-decoupled P-31 NMR 2D chemical shift correlation spectroscopy, impart stringent constraints to the O-18-isotope distributions over the whole oligophosphate moiety. The multiple deduced correlations of isotopic labels enable determination of site-specific fractional isotope enrichments and unravel the isotopologue statistics. This approach ensures accurate determination of O-18-labeling rates of phosphometabolites, critical in biochemical energy conversion and metabolic flux transmission. The biological usefulness of the J-decoupled P-31 NMR 2D chemical shift correlation maps was validated on adenosine tri-phosphate fractionally O-18 labeled in perfused mammalian hearts.