Polycystic ovary syndrome in adolescents: Q-TOF LC/MS analysis of human plasma metabolome


ÇELEBİER M. , KAPLAN O. , Özel Ş., Engin-Üstün Y.

Journal of Pharmaceutical and Biomedical Analysis, vol.191, 2020 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 191
  • Publication Date: 2020
  • Doi Number: 10.1016/j.jpba.2020.113543
  • Title of Journal : Journal of Pharmaceutical and Biomedical Analysis
  • Keywords: Polycystic ovary syndrome, Adolescents, Metabolomics, Q-TOF LC/MS, XCMS, MetaboAnalyst

Abstract

Polycystic ovary syndrome (PCOS) is a hormonal disorder common among women of reproductive age. Women with PCOS may have infrequent or prolonged menstrual periods or excess male hormone levels. Metabolomics provide information on early biochemical changes in patients. Our aim was to find potential biomarkers on metabolome level to notice PCOS in adolescents and propose treatment opportunities based on our findings on metabolome level. In this study, Q-TOF LC/MS based analysis of the plasma samples of 15 healthy adolescents as control group (Group C) were compared with the plasma samples of 15 adolescents having PCOS (Group T). Raw chromatograms were processed on XCMS using Isotopologue Parameter Optimization (IPO) to optimize XCMS parameters. Finally, 2288 peaks were found but 84 of them had fold changes >1.5 based on normalized peak areas and they were statistically different (p < 0.05) between the groups. These peaks were subjected to MetaboAnalyst 4.0 MS Peaks to Pathways utility for putative identification. The final list based on putative identification were evaluated through a clinical perspective and the statistically proved variation on the metabolite profiles of Group T and Group C presented that PCOS directly affected the lipid metabolism in the body or occurred as a result of a deformation in the lipid metabolism. Lower amount of Gamma-Tocopherol and higher amount of Coenzyme Q9, which is a product of incomplete Coenzyme Q10 biosynthesis, in the plasma samples of adolescent PCOS patients encouraged us to suggest larger randomized placebo controlled studies for Gamma-Tocopherol and Coenzyme Q10 supplements on the disease situation since our findings on metabolome level were in an accordance with the previous clinical findings. (C) 2020 Elsevier B.V. All rights reserved.