A single-tube multiplex qPCR assay for mitochondria DNA (mtDNA) copy number assessment

Kilic H. B., Bulduk B. K., KOCAEFE Y. Ç.

TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, vol.44, no.6, pp.769-777, 2019 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 44 Issue: 6
  • Publication Date: 2019
  • Doi Number: 10.1515/tjb-2018-0372
  • Journal Name: TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.769-777
  • Keywords: Mitochondrial DNA copy number, Multiplex real-time quantitative PCR, Molecular beacon probes, Mitochondrial depletion, SKELETAL-MUSCLE, GENE, QUANTIFICATION, MUTATIONS, CELLS
  • Hacettepe University Affiliated: Yes

Abstract

Objective: Detection of mtDNA copy number is required for diagnosis of mtDNA depletion. Multiplex quantification of mtDNA in blood samples was claimed via normalizing to a nuclear single copy gene using qPCR. This is not possible in high mtDNA samples due to template abundance. Multiplex qPCR assays cannot be normalized to single copy sequences of the nuclear genome.