A single-tube multiplex qPCR assay for mitochondria DNA (mtDNA) copy number assessment


Kilic H. B. , Bulduk B. K. , KOCAEFE Y. Ç.

TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, vol.44, no.6, pp.769-777, 2019 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 44 Issue: 6
  • Publication Date: 2019
  • Doi Number: 10.1515/tjb-2018-0372
  • Title of Journal : TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
  • Page Numbers: pp.769-777
  • Keywords: Mitochondrial DNA copy number, Multiplex real-time quantitative PCR, Molecular beacon probes, Mitochondrial depletion, SKELETAL-MUSCLE, GENE, QUANTIFICATION, MUTATIONS, CELLS

Abstract

Objective: Detection of mtDNA copy number is required for diagnosis of mtDNA depletion. Multiplex quantification of mtDNA in blood samples was claimed via normalizing to a nuclear single copy gene using qPCR. This is not possible in high mtDNA samples due to template abundance. Multiplex qPCR assays cannot be normalized to single copy sequences of the nuclear genome.