Biodegradation of micropollutant naproxen with a selected fungal strain and identification of metabolites

ARACAGÖK Y. D., Goker H., CİHANGİR N.

ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES, cilt.72, ss.173-179, 2017 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 72
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1515/znc-2016-0162
  • Dergi Adı: ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.173-179
  • Anahtar Kelimeler: Aspergillus niger, biodegradation, fungal cytochrome P450, O-desmethylnaproxen, 7-hydroxynaproxen, naproxen, WASTE-WATER TREATMENT, ASPERGILLUS-NIGER, TRAMETES-VERSICOLOR, YARROWIA-LIPOLYTICA, LIPASE PRODUCTION, S-NAPROXEN, DEGRADATION, REMOVAL, PHARMACEUTICALS, SOIL
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Pharmaceuticals are widely used for treating human and animal diseases. Naproxen [(S) 6-methoxy-alpha-methyl-2-naphthalene acetic acid] and its sodium salt are members of the alpha-arylpropionic acid group of nonsteroidal anti-inflammatory drugs. Due to excessive usage of naproxen, this drug has been determined even in drinking water. In this study, four fungal strains Phanerochaete chrysosporium, Funalia trogii, Aspergillus niger, and Yarrowia lipolytica were investigated in terms of naproxen removal abilities. According to LC/MS data, A. niger was found the most efficient strain with 98% removal rate. Two main by-products of fungal transformation, O-desmethylnaproxen and 7-hydroxynaproxen, were identified by using LC/MS, (HNMR)-H-1, and (CNMR)-C-13. Our results showed that O-demethylation and hydroxylation of naproxen is catalyzed by cytochrome P450 enzyme system.