Selective Amplification of Plasmonic Sensor Signal for Cortisol Detection Using Gold Nanoparticles

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Yilmaz G. E., SAYLAN İNCİ Y., GÖKTÜRK BAŞAL I., YILMAZ F., DENİZLİ A.

BIOSENSORS-BASEL, vol.12, no.7, 2022 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 12 Issue: 7
  • Publication Date: 2022
  • Doi Number: 10.3390/bios12070482
  • Journal Name: BIOSENSORS-BASEL
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Applied Science & Technology Source, EMBASE, INSPEC, MEDLINE, Directory of Open Access Journals
  • Keywords: cortisol detection, gold nanoparticles, molecular imprinting, plasmonic sensor, SALIVARY CORTISOL, ELECTROCHEMICAL IMMUNOSENSOR, BIOSENSOR, POINT
  • Hacettepe University Affiliated: Yes

Abstract

Herein, gold nanoparticles (AuNP)-modified cortisol-imprinted (AuNP-MIP) plasmonic sensor was developed for signal amplification and real-time cortisol determination in both aqueous and complex solutions. Firstly, the sensor surfaces were modified with 3-(trimethoxylyl)propyl methacrylate and then pre-complex was prepared using the functional monomer N-methacryloyl-L-histidine methyl ester. The monomer solution was made ready for polymerization by adding 2-hydroxyethyl methacrylate to ethylene glycol dimethacrylate. In order to confirm the signal enhancing effect of AuNP, only cortisol-imprinted (MIP) plasmonic sensor was prepared without AuNP. To determine the selectivity efficiency of the imprinting process, the non-imprinted (AuNP-NIP) plasmonic sensor was also prepared without cortisol. The characterization studies of the sensors were performed with atomic force microscopy and contact angle measurements. The kinetic analysis of the AuNP-MIP plasmonic sensor exhibited a high correlation coefficient (R-2 = 0.97) for a wide range (0.01-100 ppb) with a low detection limit (0.0087 ppb) for cortisol detection. Moreover, the high imprinting efficiency (k ' = 9.67) of the AuNP-MIP plasmonic sensor was determined by comparison with the AuNP-NIP plasmonic sensor. All kinetic results were validated and confirmed by HPLC.