Analysis of the inflammatory process around endosseous dental implants and natural teeth: Myeloperoxidase level and nitric oxide metabolism

Tozuem T. F., AKMAN A. C., YAMALIK N., Tulunoglu I., Turkyilmaz I., KARABULUT E., ...Daha Fazla

INTERNATIONAL JOURNAL OF ORAL & MAXILLOFACIAL IMPLANTS, cilt.22, sa.6, ss.969-979, 2007 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 22 Sayı: 6
  • Basım Tarihi: 2007
  • Dergi Adı: INTERNATIONAL JOURNAL OF ORAL & MAXILLOFACIAL IMPLANTS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.969-979
  • Anahtar Kelimeler: dental implants, inflammation, myeloperoxidase, natural teeth, nitric oxide, GINGIVAL CREVICULAR FLUID, PERIODONTAL-DISEASE, AGGRESSIVE PERIODONTITIS, ENZYME-ACTIVITY, ELASTASE-LIKE, SYNTHASE, CYTOKINES, TISSUE, DESTRUCTION, NEUTROPHILS
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Purpose: The aim of the present study was to analyze the 2 molecular measures of inflammation: (1) the nitrite, an end metabolite of nitric oxide (NO) oxidation and (2) myeloperoxidase (MPO). Both are found in peri-implant sulcus fluid (PISF) of implants and gingival crevicular fluid (GCF) of natural teeth in healthy or diseased states. Materials and Methods: A total of 109 tooth or dental implant sites, either healthy/noninflamed, inflamed (Gingival Index [GI] > 0), or affected by periodontitis, were classified, and GCF/PISF samples were obtained. GCF/PISF MPO and nitrite levels were spectrophotometrically determined. For comparison of clinical parameters and PISF/GCF nitrite and MPO levels, Kruskal-Wallis analysis followed by Mann-Whitney test with Bonferroni correction was performed. Healthy/noninflamed, slightly inflamed, moderate/severely inflamed sites were also analyzed using the Kruskal-Wallis test followed by the Mann-Whitney test with Bonferroni correction. The correlation between nitrite and MPO levels and clinical inflammatory status were analyzed with Spearman's correlation coefficient. Results: Clinical parameters, including both the GCF and PISF volumes, demonstrated gradual increases with the presence of gingival/peri-implant inflammation (P <.05). Despite the higher PISF than GCF volume at healthy sites (P=.001), there were no volumetric differences at inflamed sites (P=.771). PISF from inflamed sites (P =.025) and GCF from gingivitis and periodontitis sites presented higher total MPO levels (P <.05) Thai samples from noninflamed sites. Despite the relatively stable GCF nitrite levels at healthy and diseased sites, PISF from inflamed sites had higher nitrite content than noninflamed sites (P <.05). Conclusions: The present study demonstrated the volumetric similarities of PISF and GCF in terms of response to inflammation. However, some differences between the 2 biochemical measures of inflammation and their presence in PISF and GCF were also observed. PISF is likely to have a considerable diagnostic potential for reflecting the biologic changes around load-bearing endosseous dental implants.