The effect of cannabinoids on dinitrofluorobenzene-induced experimental asthma in mice

Bozkurt T. E., Kaya Yaşar Y., Kandilci N. T., Önder S. Ç., Erdemli İ.

RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY, cilt.231, ss.7-13, 2016 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 231
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1016/j.resp.2016.05.012
  • Dergi Adı: RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.7-13
  • Anahtar Kelimeler: Cannabinoids, Airway hyperreactivity, DNFB, Non-atopic asthma, Mouse, TACHYKININ NK1 RECEPTOR, AIRWAY SMOOTH-MUSCLE, GUINEA-PIG AIRWAYS, ALLERGIC-ASTHMA, NONATOPIC ASTHMA, MOUSE MODEL, HYPERSENSITIVITY REACTION, FUNCTIONAL EXPRESSION, NONALLERGIC ASTHMA, BRONCHIAL BIOPSIES
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Cannabinoids have anti-inflammatory effects and can produce bronchodilation in the airways. We have investigated the effects of cannabinoids on tracheal hyperreactivity and airway inflammation in dinitrofluorobenzene (DNFB)-induced experimental non-atopic asthma in mice. 5-hydroxytryptamine (5-HT)-induced contraction response was enhanced while carbachol- and electrical field stimulation induced contractions, and isoprenaline-induced relaxation responses were remained unchanged in DNFB group. The increased 5-HT-induced contractions were inhibited by incubation with either atropine or tetrodotoxin. DNFB application resulted in increased macrophage number in the bronchoalveolar lavage fluid (BALF). In vivo ACEA (CB1 agonist) treatment prevented the increase in 5-HT contractions, while JWH133 (CB2 agonist) had no effect. However, neither ACEA nor JWH133 prevented the increase in macrophage number in BALF. In vitro ACEA incubation also inhibited the increase in 5-HT contraction in DNFB group. These results show that cannabinoid CBI receptor agonist can prevent tracheal hyperreactivity to 5-HT in DNFB-induced non-atopic asthma in mice. (C) 2016 Elsevier B.V. All rights reserved.