Biofunctionalization of magnetic poly(glycidyl methacrylate) microspheres with protein A: Characterization and cellular interactions

ÇAKMAK S., Guemuesderelioglu M., DENİZLİ A.

REACTIVE & FUNCTIONAL POLYMERS, vol.69, no.8, pp.586-593, 2009 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume: 69 Issue: 8
  • Publication Date: 2009
  • Doi Number: 10.1016/j.reactfunctpolym.2009.03.012
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.586-593


Monodisperse poly(glycidyl methacrylate) (m-PGMA) microspheres which show superparamagnetic behaviour were synthesized by dispersion polymerization. Bioligand protein A was covalently immobilized onto glutaraldehyde activated microspheres (3.12 mg protein A per gram of microspheres). Cell culture studies denoted that 61% of total L929 mouse fibroblasts were bound to the m-PGMA microspheres while 84% of total cells were bounding to the protein A immobilized (m-PGMA-PrA) microspheres. Interactions between m-PGMA-PrA microspheres and L929 cells were stronger than that of m-PGMA microspheres due to the non-specific interactions between protein A and cell surface. The cells interacted with m-PGMA-PrA microspheres keep their round form rather than attaching to the tissue culture polystyrene (TCPS) surface. In conclusion, this study consists a basis for the fractionation of blood lymphocytes bearing IgG antibodies on their surfaces by using protein A immobilized m-PGMA microspheres. (C) 2009 Elsevier Ltd. All rights reserved.