Biofunctionalization of magnetic poly(glycidyl methacrylate) microspheres with protein A: Characterization and cellular interactions

ÇAKMAK S., Guemuesderelioglu M., DENİZLİ A.

REACTIVE & FUNCTIONAL POLYMERS, cilt.69, sa.8, ss.586-593, 2009 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 69 Sayı: 8
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1016/j.reactfunctpolym.2009.03.012
  • Dergi Adı: REACTIVE & FUNCTIONAL POLYMERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.586-593
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Monodisperse poly(glycidyl methacrylate) (m-PGMA) microspheres which show superparamagnetic behaviour were synthesized by dispersion polymerization. Bioligand protein A was covalently immobilized onto glutaraldehyde activated microspheres (3.12 mg protein A per gram of microspheres). Cell culture studies denoted that 61% of total L929 mouse fibroblasts were bound to the m-PGMA microspheres while 84% of total cells were bounding to the protein A immobilized (m-PGMA-PrA) microspheres. Interactions between m-PGMA-PrA microspheres and L929 cells were stronger than that of m-PGMA microspheres due to the non-specific interactions between protein A and cell surface. The cells interacted with m-PGMA-PrA microspheres keep their round form rather than attaching to the tissue culture polystyrene (TCPS) surface. In conclusion, this study consists a basis for the fractionation of blood lymphocytes bearing IgG antibodies on their surfaces by using protein A immobilized m-PGMA microspheres. (C) 2009 Elsevier Ltd. All rights reserved.