Karadeniz'de doğal gaz sondaj platformundaki sondaj kesintilerinden izole edilecek mikrobiyal konsorsiyumların petrol biyoyıkım ve biyosürfektan üretim etkinliklerinin araştırılması


Bilen Özyürek S. (Executive)

TUBITAK Project, 2021 - 2022

  • Project Type: TUBITAK Project
  • Begin Date: August 2021
  • End Date: August 2022

Project Abstract

The petroleum spills occurring during exploration, production and transportation of petroleum causes wide pollution of agriculture and aquatic areas. Every year, approximately 1.3 million tons of oil spills to the sea: this causes mass deaths in sea and coastal life. Hydrocarbon pollution occurring during the offshore drilling, especially in the Black Sea and Mediterranean regions, can last for many years; it is seen that petroleum hydrocarbons cause great damage to sea creatures and the country's economy due to its highly toxic, mutagenic, and carcinogenic structure. Especially, the formation water that comes to the surface with hydrocarbons during offshore drilling and drill sections are the main pollutant sources in the sea. It is known that bioremediation methods are very advantageous compared to physical and chemical methods in the removal of the pollution. In this context, it is aimed to conduct a study titled ‘Investigation of Petroleum Biodegradation and Biosurfactant Production Efficiencies of Microbial Consortia Isolated from the Drilling Cuttings from Natural Gas Drilling Platform in the Black Sea’. As a heterogeneous mixture of underground rock, petroleum, and drilling mud; drilling cuttings are expected to contain microorganisms that are highly tolerant of the toxic effects of petroleum and drilling mud. Especially indigenous microorganisms that will be isolated from environments contaminated with petroleum hydrocarbons are predicted to be more effective in biodegradation. However, the most important disadvantage of this process is that petroleum hydrocarbons can be broken down very slowly. Although chemical surfactants that are used for the solution of this problem are important in terms of increasing the bioavailability and biodegradation of petroleum hydrocarbons; this situation appears as a cost-increasing and non-environmentally friendly approach. Therefore, it is recommended to use biological surfactants produced by live microorganisms in the treatment of petroleum pollution. In this context, it was aimed to determine petroleum biodegradation efficiencies of all bacterial strains isolated from the drilling cuttings by gravimetric analysis. Approximately 20 different bacterial strains with different metabolic capacities in petroleum biodegradation will be identified by phenotypical and genotypical methods. Hemolysis, drop-collapse, oil displacement, surface tension, emulsification index and BATH methods will be carried out to determine the presence of biosurfactant in bacterial strains with high biodegradation efficiency. It is aimed to create a total of approximately 13 consortia in two different groups with approximately 5 different bacterial strains with high biodegradation capacity. Various physiological conditions will be optimized to increase biosurfactant production in the bacterial consortium with the highest biodegradation capacity. In optimized conditions, the n-alkane fractions of petroleum will be determined by GC-MS, while SEM and FT-IR analyzes will be applied for the morphological and chemical characterization of the biosurfactant. Thus, the synergistic and antagonistic relationship between the bacterial strains and the petroleum biodegradation and biosurfactant production capacity of the consortium will also be determined. So, it is predicted that the consortium can be actively used in the bioremediation of possible petroleum pollution in the Black Sea. It can also be used in cleaning petroleum sludge in storage tanks and facilitating the transport of petroleum through pipelines. Thus, it is thought that the possible results to be achieved will make an important contribution not only to advanced bioremediation studies but also to the petroleum and natural gas industry.